Wopanga Zinthu Zogulitsa Zogulitsa ndi Fakitale |Hecin

Zopangira

Kufotokozera Kwachidule:

Kuti tikwaniritse zofuna za msika, Timapereka ntchito zopangira zinthu zopangira makina olondola kwambiri a enzymatic reaction omwe amawongolera kulondola kwa PCR.Ndife olemekezeka kukudziwitsani ma enzyme athu asanu ndi limodzi.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Tsitsani

Zogulitsa Tags

Mndandanda wazinthu:

M-MLV Reverse Transcriptase;

HP Taq DNA Polymerase;

Super HP Taq DNA Polymerase;

Gawo limodzi la RT-PCR Master Mix (ndi dNTP);

Gawo limodzi PCR Master Mix (ndi dNTP);

Yosavuta kwambiri ya SYBR®Green qPCR Master Mix (yokhala ndi dNTP).

Yankho 3: Mayankho athunthu, ukadaulo wowumitsa amaundana umapangitsa kuyenda kukhala kosavuta

OEM kupanga

Yankho 1: Kuthetsa kufunikira kwa PCR

✓M-MLV Reverse Transcriptase (RNase H-) ;
✓HP Taq DNA Polymerase;
✓Super HP Taq DNA Polymerase;

Solution2: Njira imodzi yochitira zinthu kuti ntchitoyo ikhale yosavuta

✓Chigawo chimodzi cha RT-PCR Master Mix (ndi dNTP);
✓Chigawo chimodzi cha PCR Master Mix (ndi dNTP);
✓Wosavuta kwambiri SYBR®Greenq PCR Master Mix (yokhala ndi dNTP);

Ntchito:

Poyamba, zinthu zathu zambiri za "Enzyme Series" zidapangidwa kuti zikwaniritse ntchito ya Synthesis cDNA, Common PCR amplification, Gene recombination, imawonjezera 'A' mpaka 3' kumapeto, kutsatizana kwa woyimba, PCR ya fluorescence.

Mbali

Ukadaulo wapamwamba kwambiri wowumitsa azimitsa

Kuyenda kwa miyezi itatu pa ≤ 37 ℃, zomwe zimapulumutsa ndalama zambiri zoyendera

Kuchulukitsa ndi HC800

Pezani zotsatira mkati mwa mphindi 30!

Njira zochepetsera ntchito

8-machubu Mzere (0.1/0.2 ml) wodzazidwa kale

Ubwino Woyerekeza M-MLV Reverse Transcriptase (RNase H-)

✓Popanda RNase H-ntchito
✓Chidutswa chopangidwa ≤8kb
✓Ili ndi mphamvu yowonjezera yowonjezera pansi pa 50 ℃
✓Ndi yogwirizana ndi njira imodzi ndi iwiri ya
Zotsatira za ✓RT-PCR

HP Taq DNA Polymerase

✓Kumverera kwakukulu

✓Zigawo zokongoletsedwa za PCR zimawonjezera momwe zimachitikira

Gawo limodzi la PCR Master Mix (ndi dNTP)

✓Ntchito ndiyosavuta

✓Muli ndi buku lambiri lotentha la Taq DNA polymerase

Gawo limodzi la RT-PCR Master Mix (ndi dNTP)

✓Ntchito ndiyosavuta
✓Muli ndi buku lambiri lotentha la Taq DNA polymerase ndi M-MLV Reverse Transcriptase
✓Ndi yoyenera panjira ya Taqman fulorosenti

Yosavuta kwambiri ya SYBR®Green qPCR Master Mix (yokhala ndi dNTP)

✓Ntchito ndiyosavuta
✓Muli ndi buku loyambilira lotentha la Taq DNA polymerase Chosakanizacho chitha kugwiritsidwa ntchito mwachindunji pa PCR yamphamvu yotsika kwambiri, ✓yokhala ndi chidwi kwambiri, kukhazikika komanso kudalirika.

Super HP Taq DNA Polymerase

✓Kulekerera kwakukulu kwa PCR inhibitors monga EDTA ndi heparin
✓Zigawo zokongoletsedwa za PCR zimawonjezera momwe zimachitikira
✓Ndi kukhudzika kwakukulu, kukulitsa bwino komanso tsatanetsatane