Zosavuta kwambiri za SYBR Green qPCR Master Mix

Zosavuta kwambiri za SYBR Green qPCR Master Mix

Mawu Oyamba

Super Easy SYBR Green qPCR Master Mix (yokhala ndi dNTPs,lyophilized) ndiyokonzeka kugwiritsa ntchito, lyophilized master mixforhigh-efficiency realtime PCR amplification yomwe ingagwiritsidwe ntchito pozindikira msanga zitsanzo za DNA.Zosakanizazo zikuphatikizanso zotsekeka ziwiri zoyambira zotentha za Super HP Taq DNA polymerase, PCR Buffer, MgCl2, SYBR Green dye ndi dNTPs.Ingopanganinso master mix powonjezera madzi a giredi ya PCR pamodzi ndi template yanu ndi zoyambira ku voliyumu yonse ya 20µl. Popeza kuti zinthu za PCR zopangidwa ndi lyophilized PCR Master Mix zili ndi ma overhangs a 3'-A, amatha kukhala ophatikizana mwachindunji ndi ma T-vector.Zogulitsa za RT-qPCR ndizoyenera kusanja.

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Njira zogwirira ntchito